In the framework of our internal project ‘munimux’ (short for Munich mucins), we currently aim at further optimizing and scaling-up the purification process of porcine gastric mucins. Our goal is to produce enough of those mucins to provide the scientific community with a sufficient amount of high quality mucin for research purposes – at a reasonable cost. In the long run, we would also like to push the development of novel mucin-based products for biomedical applications, e.g., mouth sprays, contact lens coatings or tribosupplements for osteoarthritis therapy. Potential partners from industry that are interested in working with us on this goal are welcome to get in touch with us.
Mucin research is one key focus of Prof. Lieleg’s lab. During his postdoc time at Harvard and MIT, he got in contact with mucin research in the lab of Prof. Katharina Ribbeck. We optimize the purification processes of mucin in terms of mucin quality and yield, and we study study the molecular principles governing the adsorption behavior of mucins to surfaces as well as protein penetration into mucin gels. Furthermore, we develop mucin-based materials and coatings as well as mucin-based lubricants.
|Purification||Ultracentrifugation, size exclusion chromatography, cross-flow diafiltration|
|Rheology||Determining gelation kinetics and viscoelastic behavior; |
Dynamic buffer exchange of hydrogels during measurements;
Linear and non-linear viscoelasticity
|Tribology||Rotational and oscillatory tribology, i.e. stationary and migrating contact geometries; |
Different sample geometries (cylinders, planar samples) and counter materials;
Lubricant volume in the range of ~1 mL/measurement
|Profilometry||Analysis of surface topographies via metrological ISO parameters|
|Microfluidics||Molecular penetration experiments into hydrogels|
|QCM-D||Molecule adsorption to surfaces|
|Microrheology||Hydrogel permeability and micro-architecture|
|Cell culture||Biocompatibility/cell viability (live/dead staining, WST)|
Several past projects critically profited from combining our mucin expertise which that of other labs. For example, the following publications were only possible as a joint effort between or group and others.